Laboratory Experiments


Course Aim

This course is a basic introductory course in Microbiology designed to provide you with

Rules of the Laboratory


Students will be required to submit written reports of their laboratory work. Each of the 7 experiments should be submitted separately one week after completion of the experiment.

There will be a short quiz associated with each experiment session which will constitute the major assessment criteria. Each quiz has to be handed in before starting the experiment.

Attendance at Laboratory classes will be recorded, and written reports will not be accepted if students have not attended the appropriate session. The Laboratory component is a very important part of the Microbiology course since it is here that you will learn to use the basic techniques of Microbiology, which may be required of you by a potential employer.

Reports should be brief and relevant. Record results of each experiment, explain what these results indicate and finally perhaps indicate whether this is what might have been expected, by referring to textbook knowledge. Do not regurgitate methods set out in the Laboratory manual or provide elaborate theoretical background information which is not directly relevant to the experiment.

Laboratory sessions will normally run for 4 hours.

TIMETABLE Session 12 3 4 5 6 Exp. 1 1.1(1.6 Exp.2 2.1 2.2 2.3 2.4 Restreak Examine Examine Examine Gram stain Exp.3 3.1 3.2 Test and Examine Examine Exp.4 4.1 Counting colonies Exp.5 5.1 5.2 Examine Examine Exp.6 6.1 Counting Colonies Exp.7 7.1(7.3

Praticals in Microbiology

Experiment 1 - Use of the Microscope, Comparison of Single Cell Organisms, Staining of Bacteria.

1.1 - Use of the Microscope

1.2 - Comparison of Single Cell Organisms

1.3 - Staining Methods

1.4 - Simple Staining with Methylene Blue

1.5 - Gram Staining of Bacteria

1.6 - Bacterial Spore Stain

Experiment 2 - Isolation of Pure Culture, Environmental Distribution of Organisms, Throat or Pharynx and Nasopharyngeal Culture, Inhibition and Destruction of Microorganisms by Antiseptic Agents.

2.1 - Isolation of Pure Culture

2.2 - Environmental Distribution of Microorganisms

2.3 - Throat or Pharynx and NasopharyngeaL Culture

2.4 - Inhibition and Destruction of Microorganisms

Experiment 3 - Exoenzymes. Anaerobic Bacteria

3.1 - Bacterial Exoenzymes

3.2- Culture Techniques for Anaerobic Bacteria

Experiment 4 - Bacterial Growth Curve

4.1 - Bacterial Growth Curve

Experiment 5 - Titration of Plaque-forming Bacteriophage, Inhibition and Killing of Organisms by Heat

5.1 - Titration of Plaque-forming Bacteriophage

5.2 - Inhibiting and Killing of Microorganisms by Heat

Experiment 6 - Bacteria in Water and Soil

6.1 - Enumeration of Bacteria in Water and Soil

Experiment 7 - Capsule Stain, Bacterial Measurement, Commercial Multitest Systems

7.1 - Capsule Stain

7.2 - Measurement of Bacteria

7.3 Demonstration of Commercial Multitest System

Appendix I
Terms Used for the Description of Colony Characteristics on Agar Medium

Shape:Circular, irregular or rhizoid colonies; "swarming" growth
Size:diameter in mm.
Elevation:flat, convex or umbonate.
Surface:smooth, or rough, dull or glistening.
Edge:entire, undulate, crenated or rhizord
Pigment:colour and diffusibility
Opacity:transparent, translucent or opaque
Structure:amorphous, granular or filamentous
Consistency:butyrous, sticky or friable
Emulsifiability:easy or difficult
Odour:absent, decided or identifiable
Effect on the medium:haemolysis, clearing, pearly halo

Growth in Broth

Degree:scanty, moderate or profuse
Turbidity:present or absent; uniform, slight or heavy
Sediment:present or absent (slight, abundant; granular, flocculent or viscid)
Surface growth:present or absent; pellicle or ring
Odour:as for agar
Pigment:as for agar

Colonial Morphology

Convenor: Dr. Bharat Patel <B.Patel@griffith.edu.au>
HTML'd by Troy Baalham
[Created: 06 Sept 1995]
[Modified: 20 May 1996]